University Hospital Bonn
Teaching Building
Lecture Hall (A010.EG.006)
Venusberg Campus 1
53127 Bonn
Learn about your Core Facility "Flow Cytometry" at the Biomedical Center II, principals and technology of Flow Cytometry and how this is relevant to your application, experimental design and analysis.
Organizer: Flow Cytometry Core Facility, UKB
Invited speaker: Thomas Bauer-Jazayeri
Program
10.00 – 10.30 Introduction: Flow Cytometry Core Facility Bonn by Andreas Dolf. Learn more about your Core Facility the Team and their expertise as well as about the available instrumentation and coupled analysis possibilities.
10.30 – 12.00 Flow Cytometry:
What’s in the Box; Principals and technology of Flow Cytometry and how this is relevant to your application, experimental design and analysis. This includes the instrumental set-up, optics, filters, staining, nomenclature and troubleshooting. Furthermore, we will discuss the impact and requirements of instrument settings for required sensitivity for your experiment.
12.00 – 12.45 Lunchbreak
12.45 – 14.00 How to Stain what:
Learn more about the properties of the different available dyes and how to use this information for your Panel Design in Multicolor Flow Cytometry.
14.00 – 14.15 Break
14.15 – 15.15 Data Acquisition:
Relevant and required tools to optimize quality, accuracy, precision, and sensitivity of your analysis towards your experimental requirements to receive valuable and relevant data.
15.15 – 15.30 Break
15.30 – 16.00 Analysis of the cellular Functional Phenotype by active metabolism
Phenotype MicroArrays are designed to enable a comprehensive characterization of a cell’s physiological pathways for processing nutrients and handling stress. They interrogate metabolic pathways and chemical sensitivity properties of cells, allowing scientist to discover ways to optimize, inhibit or simply compare cellular behavior and Mitochondrial activity.
16.00 – 16.30 Spectral Flow Cytometry
Spectral Flow Cytometry is a technique based on conventional Flow Cytometry where a spectrograph and multichannel detector is substituted for the traditional mirrors, optical filters and photomultiplier tubes in conventional systems. Learn more about this technology and its applications and benefits.
