Skip to main content

Dataset on maintenance of microfilariae and drug testing.

Data in brief

Authors: Abdel Jelil Njouendou, Manuel Ritter, Chi Anizette Kien, Mathias E Esum, Winston Patrick Chounna Ndongmo, Fanny Fri Fombad, Narcisse Victor T Gandjui, Flobert Njiokou, Peter Enyong, Kenneth Pfarr, Joseph Turner, Laura E Layland, Achim Hoerauf, Samuel Wanji

Endemic communities of infections have been neglected since associated pathology remains undefined. Consequently, improvements in drug therapy have also been ignored despite a large number of infected individuals in areas of Cameroon. Thus, we established an system to culture microfilariae (Mf); the transmission stage of infection. In short, we compared the ability of two renowned culture media (Dulbecco's Modified Eagle's Medium (DMEM) and Roswell Park Memorial Institute (RPMI-1640)) to sustain Mf in culture. Media were supplemented with 10% fetal bovine serum (FBS) and monkey kidney epithelial cells (LLC-MK) were used as feeder cells. As readout we assessed Mf survival and motility using a standardised microscopy assessment strategy. Moreover, this culture system was used to test susceptibility levels of microfilariae to different chemotherapeutic agents. Parasite motility was scored daily using a graded system and analysed using the average motility and area under the motility curve of Mf. These datasets were analysed and discussed in detail in the related article entitled: " maintenance of microfilariae and its relevance for drug screening" [1].

© 2019 The Authors.

PMID: 31886361

Participating cluster members