Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).
European journal of immunology
Authors:
Andrea Cossarizza, Hyun-Dong Chang, Andreas Radbruch, Andreas Acs, Dieter Adam, Sabine Adam-Klages, William W Agace, Nima Aghaeepour, Mübeccel Akdis, Matthieu Allez, Larissa Nogueira Almeida, Giorgia Alvisi, Graham Anderson, Immanuel Andrä, Francesco Annunziato, Achille Anselmo, Petra Bacher, Cosima T Baldari, Sudipto Bari, Vincenzo Barnaba, Joana Barros-Martins, Luca Battistini, Wolfgang Bauer, Sabine Baumgart, Nicole Baumgarth, Dirk Baumjohann, Bianka Baying, Mary Bebawy, Burkhard Becher, Wolfgang Beisker, Vladimir Benes, Rudi Beyaert, Alfonso Blanco, Dominic A Boardman, Christian Bogdan, Jessica G Borger, Giovanna Borsellino, Philip E Boulais, Jolene A Bradford, Dirk Brenner, Ryan R Brinkman, Anna E S Brooks, Dirk H Busch, Martin Büscher, Timothy P Bushnell, Federica Calzetti, Garth Cameron, Ilenia Cammarata, Xuetao Cao, Susanna L Cardell, Stefano Casola, Marco A Cassatella, Andrea Cavani, Antonio Celada, Lucienne Chatenoud, Pratip K Chattopadhyay, Sue Chow, Eleni Christakou, Luka Čičin-Šain, Mario Clerici, Federico S Colombo, Laura Cook, Anne Cooke, Andrea M Cooper, Alexandra J Corbett, Antonio Cosma, Lorenzo Cosmi, Pierre G Coulie, Ana Cumano, Ljiljana Cvetkovic, Van Duc Dang, Chantip Dang-Heine, Martin S Davey, Derek Davies, Sara De Biasi, Genny Del Zotto, Gelo Victoriano Dela Cruz, Michael Delacher, Silvia Della Bella, Paolo Dellabona, Günnur Deniz, Mark Dessing, James P Di Santo, Andreas Diefenbach, Francesco Dieli, Andreas Dolf, Thomas Dörner, Regine J Dress, Diana Dudziak, Michael Dustin, Charles-Antoine Dutertre, Friederike Ebner, Sidonia B G Eckle, Matthias Edinger, Pascale Eede, Götz R A Ehrhardt, Marcus Eich, Pablo Engel, Britta Engelhardt, Anna Erdei, Charlotte Esser, Bart Everts, Maximilien Evrard, Christine S Falk, Todd A Fehniger, Mar Felipo-Benavent, Helen Ferry, Markus Feuerer, Andrew Filby, Kata Filkor, Simon Fillatreau, Marie Follo, Irmgard Förster, John Foster, Gemma A Foulds, Britta Frehse, Paul S Frenette, Stefan Frischbutter, Wolfgang Fritzsche, David W Galbraith, Anastasia Gangaev, Natalio Garbi, Brice Gaudilliere, Ricardo T Gazzinelli, Jens Geginat, Wilhelm Gerner, Nicholas A Gherardin, Kamran Ghoreschi, Lara Gibellini, Florent Ginhoux, Keisuke Goda, Dale I Godfrey, Christoph Goettlinger, Jose M González-Navajas, Carl S Goodyear, Andrea Gori, Jane L Grogan, Daryl Grummitt, Andreas Grützkau, Claudia Haftmann, Jonas Hahn, Hamida Hammad, Günter Hämmerling, Leo Hansmann, Goran Hansson, Christopher M Harpur, Susanne Hartmann, Andrea Hauser, Anja E Hauser, David L Haviland, David Hedley, Daniela C Hernández, Guadalupe Herrera, Martin Herrmann, Christoph Hess, Thomas Höfer, Petra Hoffmann, Kristin Hogquist, Tristan Holland, Thomas Höllt, Rikard Holmdahl, Pleun Hombrink, Jessica P Houston, Bimba F Hoyer, Bo Huang, Fang-Ping Huang, Johanna E Huber, Jochen Huehn, Michael Hundemer, Christopher A Hunter, William Y K Hwang, Anna Iannone, Florian Ingelfinger, Sabine M Ivison, Hans-Martin Jäck, Peter K Jani, Beatriz Jávega, Stipan Jonjic, Toralf Kaiser, Tomas Kalina, Thomas Kamradt, Stefan H E Kaufmann, Baerbel Keller, Steven L C Ketelaars, Ahad Khalilnezhad, Srijit Khan, Jan Kisielow, Paul Klenerman, Jasmin Knopf, Hui-Fern Koay, Katja Kobow, Jay K Kolls, Wan Ting Kong, Manfred Kopf, Thomas Korn, Katharina Kriegsmann, Hendy Kristyanto, Thomas Kroneis, Andreas Krueger, Jenny Kühne, Christian Kukat, Désirée Kunkel, Heike Kunze-Schumacher, Tomohiro Kurosaki, Christian Kurts, Pia Kvistborg, Immanuel Kwok, Jonathan Landry, Olivier Lantz, Paola Lanuti, Francesca LaRosa, Agnès Lehuen, Salomé LeibundGut-Landmann, Michael D Leipold, Leslie Y T Leung, Megan K Levings, Andreia C Lino, Francesco Liotta, Virginia Litwin, Yanling Liu, Hans-Gustaf Ljunggren, Michael Lohoff, Giovanna Lombardi, Lilly Lopez, Miguel López-Botet, Amy E Lovett-Racke, Erik Lubberts, Herve Luche, Burkhard Ludewig, Enrico Lugli, Sebastian Lunemann, Holden T Maecker, Laura Maggi, Orla Maguire, Florian Mair, Kerstin H Mair, Alberto Mantovani, Rudolf A Manz, Aaron J Marshall, Alicia Martínez-Romero, Glòria Martrus, Ivana Marventano, Wlodzimierz Maslinski, Giuseppe Matarese, Anna Vittoria Mattioli, Christian Maueröder, Alessio Mazzoni, James McCluskey, Mairi McGrath, Helen M McGuire, Iain B McInnes, Henrik E Mei, Fritz Melchers, Susanne Melzer, Dirk Mielenz, Stephen D Miller, Kingston H G Mills, Hans Minderman, Jenny Mjösberg, Jonni Moore, Barry Moran, Lorenzo Moretta, Tim R Mosmann, Susann Müller, Gabriele Multhoff, Luis Enrique Muñoz, Christian Münz, Toshinori Nakayama, Milena Nasi, Katrin Neumann, Lai Guan Ng, Antonia Niedobitek, Sussan Nourshargh, Gabriel Núñez, José-Enrique O'Connor, Aaron Ochel, Anna Oja, Diana Ordonez, Alberto Orfao, Eva Orlowski-Oliver, Wenjun Ouyang, Annette Oxenius, Raghavendra Palankar, Isabel Panse, Kovit Pattanapanyasat, Malte Paulsen, Dinko Pavlinic, Livius Penter, Pärt Peterson, Christian Peth, Jordi Petriz, Federica Piancone, Winfried F Pickl, Silvia Piconese, Marcello Pinti, A Graham Pockley, Malgorzata Justyna Podolska, Zhiyong Poon, Katharina Pracht, Immo Prinz, Carlo E M Pucillo, Sally A Quataert, Linda Quatrini, Kylie M Quinn, Helena Radbruch, Tim R D J Radstake, Susann Rahmig, Hans-Peter Rahn, Bartek Rajwa, Gevitha Ravichandran, Yotam Raz, Jonathan A Rebhahn, Diether Recktenwald, Dorothea Reimer, Caetano Reis e Sousa, Ester B M Remmerswaal, Lisa Richter, Laura G Rico, Andy Riddell, Aja M Rieger, J Paul Robinson, Chiara Romagnani, Anna Rubartelli, Jürgen Ruland, Armin Saalmüller, Yvan Saeys, Takashi Saito, Shimon Sakaguchi, Francisco Sala-de-Oyanguren, Yvonne Samstag, Sharon Sanderson, Inga Sandrock, Angela Santoni, Ramon Bellmàs Sanz, Marina Saresella, Catherine Sautes-Fridman, Birgit Sawitzki, Linda Schadt, Alexander Scheffold, Hans U Scherer, Matthias Schiemann, Frank A Schildberg, Esther Schimisky, Andreas Schlitzer, Josephine Schlosser, Stephan Schmid, Steffen Schmitt, Kilian Schober, Daniel Schraivogel, Wolfgang Schuh, Thomas Schüler, Reiner Schulte, Axel Ronald Schulz, Sebastian R Schulz, Cristiano Scottá, Daniel Scott-Algara, David P Sester, T Vincent Shankey, Bruno Silva-Santos, Anna Katharina Simon, Katarzyna M Sitnik, Silvano Sozzani, Daniel E Speiser, Josef Spidlen, Anders Stahlberg, Alan M Stall, Natalie Stanley, Regina Stark, Christina Stehle, Tobit Steinmetz, Hannes Stockinger, Yousuke Takahama, Kiyoshi Takeda, Leonard Tan, Attila Tárnok, Gisa Tiegs, Gergely Toldi, Julia Tornack, Elisabetta Traggiai, Mohamed Trebak, Timothy I M Tree, Joe Trotter, John Trowsdale, Maria Tsoumakidou, Henning Ulrich, Sophia Urbanczyk, Willem van de Veen, Maries van den Broek, Edwin van der Pol, Sofie Van Gassen, Gert Van Isterdael, René A W van Lier, Marc Veldhoen, Salvador Vento-Asturias, Paulo Vieira, David Voehringer, Hans-Dieter Volk, Anouk von Borstel, Konrad von Volkmann, Ari Waisman, Rachael V Walker, Paul K Wallace, Sa A Wang, Xin M Wang, Michael D Ward, Kirsten A Ward-Hartstonge, Klaus Warnatz, Gary Warnes, Sarah Warth, Claudia Waskow, James V Watson, Carsten Watzl, Leonie Wegener, Thomas Weisenburger, Annika Wiedemann, Jürgen Wienands, Anneke Wilharm, Robert John Wilkinson, Gerald Willimsky, James B Wing, Rieke Winkelmann, Thomas H Winkler, Oliver F Wirz, Alicia Wong, Peter Wurst, Jennie H M Yang, Juhao Yang, Maria Yazdanbakhsh, Liping Yu, Alice Yue, Hanlin Zhang, Yi Zhao, Susanne Maria Ziegler, Christina Zielinski, Jakob Zimmermann, Arturo Zychlinsky
These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.