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induction of trained immunity in adherent human monocytes.

STAR protocols

Authors: Jorge Domínguez-Andrés, Rob J W Arts, Siroon Bekkering, Harsh Bahrar, Bastiaan A Blok, L Charlotte J de Bree, Mariolina Bruno, Özlem Bulut, Priya A Debisarun, Helga Dijkstra, Jéssica Cristina Dos Santos, Anaísa V Ferreira, Daniela Flores-Gomez, Laszlo A Groh, Inge Grondman, Leonie Helder, Cor Jacobs, Liesbeth Jacobs, Trees Jansen, Gizem Kilic, Viola Klück, Valerie A C M Koeken, Heidi Lemmers, Simone J C F M Moorlag, Vera P Mourits, Jelmer H van Puffelen, Katrin Rabold, Rutger J Röring, Diletta Rosati, Helin Tercan, Julia van Tuijl, Jessica Quintin, Reinout van Crevel, Niels P Riksen, Leo A B Joosten, Mihai G Netea

A growing number of studies show that innate immune cells can undergo functional reprogramming, facilitating a faster and enhanced response to heterologous secondary stimuli. This concept has been termed "trained immunity." We outline here a protocol to recapitulate this using adherent monocytes from consecutive isolation of peripheral blood mononuclear cells. The induction of trained immunity and the associated functional reprogramming of monocytes is described in detail using β-glucan (from ) and Bacillus Calmette-Guérin as examples. For complete details on the use and execution of this protocol, please refer to Repnik et al. (2003) and Bekkering et al. (2016).

© 2021 The Author(s).

PMID: 33718890

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