Prof. Dr. Volker Busskamp
University Eye Clinic
volker.busskamp@uni-bonn.de View member: Prof. Dr. Volker Busskamp
Frontiers in genome editing
Gene activation with the CRISPR-Cas system has great implications in studying gene function, controlling cellular behavior, and modulating disease progression. In this review, we survey recent studies on targeted gene activation and multiplexed screening for inducing neuronal differentiation using CRISPR-Cas transcriptional activation (CRISPRa) and open reading frame (ORF) expression. Critical technical parameters of CRISPRa and ORF-based strategies for neuronal programming are presented and discussed. In addition, recent progress on applications of CRISPRa to the nervous system are highlighted. Overall, CRISPRa represents a valuable addition to the experimental toolbox for neuronal cell-type programming.
Copyright © 2021 Liu, Striebel, Pasquini, Ng, Khoshakhlagh, Church and Busskamp.
PMID: 34713262