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Next generation genetically encoded fluorescent sensors for serotonin.

Nature communications

Authors: Martin Kubitschke, Monika Müller, Lutz Wallhorn, Mauro Pulin, Manuel Mittag, Stefan Pollok, Tim Ziebarth, Svenja Bremshey, Jill Gerdey, Kristin Carolin Claussen, Kim Renken, Juliana Groß, Pascal Gneiße, Niklas Meyer, J Simon Wiegert, Andreas Reiner, Martin Fuhrmann, Olivia Andrea Masseck

We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging.

© 2022. The Author(s).

PMID: 36473867

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