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Optogenetic Control of Human Stem Cell-Derived Neurons.

Methods in molecular biology (Clifton, N.J.)

Authors: Rouhollah Habibey, Johannes Striebel, Kritika Sharma, Volker Busskamp

Spontaneous and optogenetically evoked activities of human induced pluripotent stem cell (hiPSC)-derived neurons can be assessed by patch clamp and multi-electrode array (MEA) electrophysiology. Optogenetic activation of these human neurons facilitates the characterization of their functional properties at the single neuron and circuit level. Here we showcase the preparation of hiPSC-derived neurons expressing optogenetic actuators, in vitro optogenetic stimulation and simultaneous functional recordings using patch clamp and MEA electrophysiology.

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PMID: 35857237

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